Protein NMR Techniques (Methods in Molecular Biology #831) (Paperback)

Description

Table of Contents

1. A Novel Bacterial Expression Method with Optimized Parameters for Very High Yield Production of Triple-labeled Proteins

2 Isotopic Labeling of Heterologous Proteins in the Yeast Pichia pastoris and Kluyveromyces lactis

3. Isotope Labeling in Insect Cells

4. Isotope Labeling in Mammalian Cells

5. Cell-free Protein Production for NMR Studies

6. Cell-free Membrane Protein Expression for Solid-state NMR

7. Expression and Purification of Src-family Kinases for Solution NMR Studies

8. NMR Studies of Large Protein Systems

9. Protein Dynamics by 15N Nuclear Magnetic Relaxation

10. Bacterial Production and Solution NMR Studies of a Viral Membrane Ion Channel

11. Preparation of the Modular Multi-Domain Protein RPA for Study by NMR Spectroscopy

12. NMR Studies of Protein-RNA Interactions

13. Preparation and Optimization of Protein-DNA Complexes Suitable for Detailed NMR Studies

15. In-cell NMR Spectroscopy in Escherichia coli

17. Solid-State NMR Spectroscopy of Protein Complexes

18. Synthesis, Purification and Characterization of Single Helix Membrane Peptides and Proteins for NMR Spectroscopy

19. Assignment of Backbone Resonances in a Eukaryotic Protein Kinase - ERK2

20. Electrostatics of Hydrogen Exchange for Analyzing Protein Flexibility

21. Fast Protein Backbone NMR Resonance Assignment using the BATCH Strategy

23. ARIA for Solution and Solid-state NMR Protein NMR Techniques

24. Determining Protein Dynamics from 15N Relaxation Data by using DYNAMICS